ASSESSMENT OF NUCLEOLLUS PARAMETERS DEPENDING ON THE GENOTYPE OF SHEEP
УДК: 636.32.38.082.12:575.11 DOI: 10.31588/2413_4201_1883_2_254_207
Keywords:
nucleoli, sheep, morphometry, nucleolar organizers regions (NOR), genotypeAbstract
In the course of the research, the morphometric and photometric parameters of the NOR of sheep were studied depending on their genotype. Differences in the number of nucleoli in the cells between the studied groups of animals were revealed. In purebred Romanov sheep (group I), the maximum number of NORs was observed in the amount of 11 pcs, the minimum — 1 pcs. While in group II these figures were 8 pcs and 1 pc, respectively. The coefficient of variation of the average value of the nucleolus area reached 52.8 %, the indicator of the average NOR area of sheep, regardless of the genotype, was 376.87 µm². The difference in the average background density was statistically significant between the animals of groups I and II, and in Romanov sheep it was less by 5.3 % (P<0.05). The density of the area free from NOR in sheep of the Romanov breed was less than in animals of group III (hybrids with the genotype 1/16 argali x 9/16 Romanov x 6/16 mouflon). The difference between the groups was 6.6 % (P<0.05). Sheep of group IV (hybrids with the genotype ¼ argali x ¼ Romanov x 2/4 katadin) were also significantly inferior to groups II and III, respectively, by 6.8 and 8.1 % (P<0.05). The results of the study allow us to conclude that the genotype of animals should be considered as one of the main factors influencing the morphometric, photometric and quantitative parameters of the nucleoli.
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