STUDY OF ASSOCIATIONS OF KCNB1 GENE POLYMORPHISM WITH MILK PRODUCTIVITY AND MILK QUALITY WITH THE INCLUDING “KLYUVER PRO” YEAST ADDITIVE IN THE DIET
DOI 10.31588/2413_4201_1883_4_264_108 УДК 636.082
Abstract
High-producing dairy cattle are particularly susceptible to metabolic disorders and nutrition-related diseas
es, as their physiology is primarily focused on milk synthesis, even in the face of nutrient deficiencies. This is due to the
fact that years of selection for milk yield have led to the development of a lactation dominant system, in which the body
devotes a significant portion of its resources to milk production, often to the detriment of other systems. Including yeast
probiotics, which have the ability to absorb oxygen in the rumen, in the diet promotes the formation of a favorable anaero
bic environment for the normal flora of the rumen. Genetic factors play a significant role in the stable establishment of
productive traits in cattle. The aim of this study was to investigate the relationship between the KCNB1 gene polymor
phism (g.78216220G>A) and milk productivity in Holstein cows fed the probiotic yeast supplement “KLYUVER PRO”,
based on Kluyveromyces marxianus yeast strain. The study involved 120 lactating cows, grouped into 60 cows at Lenin
Agricultural Breeding Farm in Atninsky District of the Republic of Tatarstan. The experimental group was additionally fed
20 g of “KLYUVER PRO” per cow per day for 60 days. The study revealed that, compared to the control herd, the group
of cows with the KCNB1 AA genotype that received the supplement demonstrated an average daily milk yield improve
ment of 6.94%, milk protein yield of 10.73%, and the sum of fat and protein by 11.32%, including a significant increase in
milk fat by 11.86% (p≤0.05). In the same comparison, the herd with the KCNB1 GG genotype outperformed the control
herd in average daily milk yield by 5.96%, milk fat by 9.01%, the sum of fat and protein by 8.83%, and significantly ex
ceeded the control herd in milk protein yield by 8.64% (p≤0.05).
References
Литература
Анализ нарушений обмена веществ у высокоудойных коров / В. А. Мищенко, А. В. Мищенко, И. В.
Ермилов [и др.] // Ветеринария Кубани. 2012. № 6. С. 15-17. EDN PNFBVT.
Использование пробиотиков для коррекции нарушения обмена веществ у коров / О. В. Ковалева, Н.
В. Санникова, О. В. Шулепова [и др.] // Кормление сельскохозяйственных животных и кормопроизводство.
№ 5(190). С. 47-53. https://doi.org/ 10.33920/sel-05-2105-06. EDN ACAEFO.
Молочная продуктивность и качество молока высокопродуктивных коров при использовании иннова
ционной дрожжевой добавки «КЛЮВЕР ПРО» / Н. Д. Чевтаева, И. Т. Бикчантаев, Ф. Ф. Зиннатов [и др.] //
Аграрный научный журнал. 2025. № 5. С. 72-78. https://doi.org/10.28983/asj.y2025i5pp72-78.
Kluyveromyces marxianus as a Probiotic Yeast: A Mini-review. / A. Homayouni, A. Azizi, P. Oroojzadeh [et
al.]
//
Current
Nutrition
& Food Science. 2020. Vol. 16. No 8. https://doi.org/
2174/1573401316666200217113230.
Effect of Supplemental Kluyveromyces marxianus and Pichia kudriavzevii on Aflatoxin M1 Excretion in
Milk of Lactating Dairy Cows / M. Intanoo, M. B. Kongkeitkajorn, W. Suriyasathaporn [et al.] // Animals (Basel).
Vol. 4. № 10. Р. 709. https://doi.org/10.3390/ani10040709.
Дрожжевые микроорганизмы как пробиотики и источник белка / М. Г. Саубенова, Е А. Олейникова,
А. Ж. Алыбаева [и др.] // Микробиология және вирусология. 2023. Т. 42. №3. С. 13-18. https://
doi.org/10.53729/MV-AS.2023.03.01.
Перспективы использования поликомпонентной буферной смеси с включением дрожжей в кормлении
коров / Д. М. Фертикова, Е. М. Кислякова, Н. В. Селезнева [и др.] // Вестник КрасГАУ. 2022. Т. 180. № 3. С.
-112. – https://doi.org/ 10.36718/1819-4036-2022-3-107-112.
Чевтаева Н. Д., Зиннатов Ф. Ф., Бикчантаев И. Т. Влияние полиморфизма гена PIT-1 на продуктивные
качества высокоудойных коров при скармливании пробиотической добавки «КЛЮВЕР ПРО» // Приоритет
ные направления повышения эффективности, конкурентоспособности и устойчивости аграрной отрасли:
Материалы международной научной конференции, посвященной 105-летию ТатНИИСх ФИЦ КазНЦ РАН,
Казань, 10–11 июля 2025 года. Санкт-Петербург: ООО Издательский дом "Сциентиа", 2025. С. 485-487.
https://doi.org/ 10.32415/scientia_978-5-907902-43-5(485-487. EDN DKXEGP.
Genomewide Association Analyses of Lactation Persistency and Milk Production Traits in Holstein Cattle
Based on Imputed Whole-Genome Sequence Data / V. B. Pedrosa, F. S. Schenkel, S. Y. Chen [et al.] // Genes. 2021.
Vol. 112. No 11. Р. 1830. https://doi.org/ 10.3390/genes12111830.
Association of udder traits with single nucleotide polymorphisms in crossbred Bos indicus–Bos taurus
cows / M. W. Tolleson, C. A. Gill, A. D. Herring [et al.] // Journal of Animal Science. 2017. Vol. 95. No 6. Р. 2399
https://doi.org/ 10.2527/jas.2017.1475.
Lopdell T. J. Using QTL to Identify Genes and Pathways Underlying the Regulation and Production of Milk
Components in Cattle // Animals. 2023. Vol. 13. No 5. Р. 911. https://doi.org/ 10.3390/ani13050911.
Novel polymorphisms of the KCNB1 gene and their association with production traits in Indian Sahiwal
cattle / R. Kumari, R. Kumar, B. Sinha [et al.] // The Indian Journal of Animal Sciences. 2022. Vol. 92. No 12.
https://doi.org/ 10.56093/ijans.v92i12.117454.
References
Mishchenko VA, Mishchenko AV, Ermilov IV. [Analysis of metabolic disorders in high-yielding cows]. Veterinariya
Kubani. 2012; 6. 15-17 p. EDN: PNFBVT.
Kovaleva OV, Sannikova NV, Shulepova OV. [The use of probiotics for the correction of metabolic disorders in
cows]. Kormlenie selskokhozyaystvennykh zhivotnykh i kormoproizvodstvo. 2021; 5(190). 47-53 p. https://
doi.org/10.33920/sel-05-2105-06. EDN: ACAEFO.
Chevtaeva ND, Bikchantaev IT, Zinnatov FF. [Milk productivity and milk quality of highly productive cows when
using the innovative yeast additive “KLYUVER PRO”]. Agrarnyy nauchnyy zhurnal. 2025; 5. 72-78 p. https://
doi.org/10.28983/asj.y2025i5pp72-78.
Homayouni A, Azizi A, Oroojzadeh P. Kluyveromyces marxianus as a probiotic yeast: a mini-review. Current Nutri
tion & Food Science. 2020; Vol.16. No.8. https://doi.org/ 10.2174/1573401316666200217113230.
Intanoo M, Kongkeitkajorn MB, Suriyasathaporn W. Effect of supplemental Kluyveromyces marxianus and Pichia
kudriavzevii on aflatoxin M1 excretion in milk of lactating dairy cows. Animals (Basel). 2020; Vol.4. 10. 709 p. https://
doi.org/10.3390/ani10040709.
Saubenova MG, Oleynikova EA, Alybaeva AZh. [Yeast microorganisms as probiotics and a source of protein].
Mikrobiologiya zhane virusologiya. 2023; Vol.42. 3. 13-18 p. https://doi.org/10.53729/MV-AS.2023.03.01.
Fertikova DM, Kislyakova EM, Selezneva NV. [Prospects for the use of a multicomponent buffer mixture with the
inclusion of yeast in cow feeding]. Vestnik KrasGAU. 2022; Vol.180. 3. 107-112 p. https://doi.org/ 10.36718/1819-4036
-3-107-112.
Chevtaeva ND, Zinnatov FF, Bikchantaev IT. [Influence of PIT-1 gene polymorphism on the productive qualities of
high-yielding cows when fed “KLYUVER PRO” probiotic supplement. Priority areas for improving the efficiency, com
petitiveness and sustainability of agricultural sector]. Materialy mezhdunarodnoy nauchnoy konferentsii, posvyashchennoy
-letiyu TatNIISkh FITs KazNTs RAN, Kazan, 10-11 iyulya 2025 goda. Saint-Petersburg: OOO Izdatelskiy dom Stsien
tia. 2025; 485-487 p. https://doi.org/ 10.32415/scientia_978-5-907902-43-5(485-487. EDN DKXEGP.
Pedrosa VB, Schenkel FS, Chen SY. Genomewide association analyses of lactation persistency and milk production
traits in Holstein cattle based on imputed whole-genome sequence data. Genes. 2021; Vol.112. No.11. 1830 p. https://
doi.org/ 10.3390/genes12111830.
Tolleson MW, Gill CA, Herring AD. Association of udder traits with single nucleotide polymorphisms in crossbred
Bos indicus – Bos taurus cows. Journal of Animal Science. 2017; Vol.95. No.6. 2399-2407 p. https://doi.org/ 10.2527/
jas.2017.1475.
Lopdell TJ. Using QTL to identify genes and pathways underlying the regulation and production of milk compo
nents in cattle. Animals. 2023; Vol.13. No.5. 911 p. https://doi.org/ 10.3390/ani13050911.
Kumari R, Kumar R, Sinha B. Novel polymorphisms of the KCNB1 gene and their association with production
traits in Indian Sahiwal cattle. The Indian Journal of Animal Sciences. 2022; Vol.92. No.12. https://doi.org/ 10.56093/
ijans.v92i12.117454.
